Behavioural responses to separation from human companion in the domestic cat: A survey-based study.

The domestic cat is known to react to social separation, though the conceptual relationship between separation-related behaviours outside of a clinical context has not been described in detail. We did an online survey on participants who had cats in their households (Nparticipants=114; Ncats=133) and asked them to evaluate the frequency of 12 behavioural elements associated with social separation from human companions on a 5- point Likert Scale. We performed two dimensionality reduction techniques (component and factor analyses) to assess whether the specified behaviours related to social separation belonged to the same axis. We found four distinct dimensions instead of one: (a) reactivity towards companion departure cues, (b) protest behaviour towards inaccessibility, (c) unusual elimination behaviour, and (d) negative responses following social separation. Our findings suggest a manifestation of different motivational states rather than a single, separation-related construct. Future studies would benefit from a careful evaluation of the separation-related behaviours in a multi-measure context to improve the accuracy of ethological classifications.

Evidence for Male Horn Dimorphism and Related Pronotal Shape Variation in Copris lunaris (Linnaeus, 1758) (Coleoptera: Scarabaeidae, Coprini).

Male horn dimorphism is a rather common phenomenon in dung beetles, where some adult individuals have well-developed head horns (i.e., major males), while others exhibit diminished horn length (i.e., minor males). We focused on horn dimorphism and associated head and pronotum shape variations in Copris lunaris. We examined the allometric relationship between horn length (i.e., cephalic and pronotal horns) and maximum pronotum width (as index of body size) by fitting linear and sigmoidal models for both sexes. We then asked whether head and pronotum shape variations, quantified using the geometric morphometric approach, contributed to this allometric pattern. We found that female cephalic and pronotal horn growth showed a typical isometric scaling with body size. Horn length in males, however, exhibited sigmoidal allometry, where a certain threshold in body size separated males into two distinct morphs as majors and minors. Interestingly, we highlighted the same allometric patterns (i.e., isometric vs. sigmoidal models) by scaling horn lengths with pronotum shape, making evident that male horn dimorphism is not only a matter of body size. Furthermore, the analysis of shape showed that the three morphs had similar heads, but different pronota, major males showing a more expanded, rounded pronotum than minor males and females. These morphological differences in C. lunaris can ultimately have important functional consequences in the ecology of this species, which should be explored in future work.

The effect of social context on measures of boldness: Zebra finches (Taeniopygia guttata) are bolder when housed individually.

Boldness can be quantified as latency to access resources in novel contexts. Although social interactions influence boldness, especially in species such as zebra finches (Taeniopygia guttata) that are sociable and live in groups, the boldness of individuals is generally characterized in isolation. The objective of this study was to examine how proximity of and familiarity with flock members influenced boldness, which is often assumed to be stable across contexts. We measured boldness as the latency of adult zebra finches to forage when a novel object was within a food dish in five social contexts: (1) social isolation, (2) adjacent to novel flock, (3) adjacent to familiar flock, (4) within novel flock, (5) within familiar flock. Our measure of boldness showed individual consistency across social contexts. However, when mean response values across different social contexts were compared, individuals were bolder (i.e. had lower latencies) when housed and tested individually, regardless of the presence or absence of a flock nearby. We found no evidence of sex, dominance rank, or flock members' boldness on the boldness of a focal bird. Our study deepens understanding of the influence of the social environment on boldness in zebra finches by demonstrating an influence of physical proximity. We encourage continued work examining boldness in different social contexts in zebra finches, as rigorous work in one system may help us understand the factors that influence the relative stability or flexibility of this trait.

Beeswax corticosterone implants produce long-term elevation of plasma corticosterone and influence condition.

Glucocorticoids can play a critical role in modulating life-history trade-offs. However, studying the effects of glucocorticoids on life-history often requires experimentally elevating plasma glucocorticoid concentrations for several weeks within normal physiological limits and without repeated handling of the animal. Recently, implants made of beeswax and testosterone (T) were shown to have release dynamics superior to some currently available T implants, and these beeswax implants dissolved, eliminating the need to recapture the animal. We evaluated the utility of beeswax implants containing four different dosages of corticosterone (CORT; the primary glucocorticoid in birds) and their effect on several condition indices in a captive colony of zebra finches (Taeniopygia guttata). The three implants with the greatest CORT doses (0.05, 0.1, and 0.5mg) produced spikes in plasma CORT concentrations 20h after treatment, but were within the limits that zebra finches may normally experience. The 0.5mg CORT implant elevated plasma CORT between typical baseline and restraint stress levels reported in other studies of zebra finches for the entire 35day experiment. Birds in the 0.5mg implant group were heavier, had greater furcular fat scores, and had lower hematocrit than birds in the control and other CORT implant groups. Beeswax CORT implants are a low cost method of elevating plasma CORT for a prolonged time. Furthermore, because there is no need to remove these implants at the end of a study, this method may be amenable to studies of free-ranging animals.